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KMID : 0903620010420020210
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Journal of the Korean Society for Horticultural Science
2001 Volume.42 No. 2 p.210 ~ p.214
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Improvement of Multiplication Rate through Organ - Callus Mixture Culture in Gladiolus ´ Topaz ´
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Abstract
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This study was performed to establish the optimal culture conditions for producing multiple shoots from the organ-callus mixture derived from callus by using the liquid shaking culture in gladiolus ´Topaz´. Obtaining the organ-callus mixture from callus was very effective when it was grown in liquid shaking medium containing 0.05 ㎎·L^(-1) BA. Shoot regeneration took place from organ-callus mixture when it was traps-cultured in solid medium containing 0.1 ㎎·L^(-1) BA at 30℃ under 16 hours daylength. Based on microscopic examination, meristemoid was clearly observed, which was then developed into shoot primordia in organ-callus mixture. On a basis of these results, it was possible to establish the model for a mass production of multiple shoots from organ-callus mixtures.
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